ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Comparison of a Serological Method, a Bacteriological Method and 16S rRNA Brucella canis PCR for Canine Brucellosis Diagnosis)

ผู้เขียน:ดร.จตุพร รัตนศรีสมพร (หนูสุด), รองศาสตราจารย์, ดร.เกษกนก ศิรินฤมิตร, รองศาสตราจารย์, ดร.ธีระพล ศิรินฤมิตร, รองศาสตราจารย์

สื่อสิ่งพิมพ์:pdf

Abstract

The objective of the study was to evaluate the sensitivity, specificity and the positive and negative predictive value of a PCR assay for canine brucellosis diagnosis using 16S rRNA specific primers compared to serology, 2 mercaptoethanol-microtiter plate agglutination tests (2ME-MPAT) and a blood culture test. A sample of 48 dogs was divided into three groups, according to the results of blood culture tests and 2ME-MPAT. Group 1was comprised of infected Brucella canis dogs, who were positive to both a blood culture test and 2ME-MPAT (n=16). Group 2 contained non-infected B. canis dogs, who were negative to both a blood culture test and 2ME-MPAT (n=16). Group 3 contained suspected infected B. canis dogs, who were negative to a blood culture test but positive to 2ME-MPAT (n=16). Samples in Groups 1 and 2 were used to calculate the diagnostic sensitivity, specificity, positive predictive value and negative predictive value of PCR and the results performed in Group 3 were also discussed. The diagnostic sensitivities and specificities of PCR were 100%. The positive and negative predictive values and accuracy of PCR were 100%. In conclusion, the results revealed that the PCR was an effective technique for the diagnosis of canine brucellosis in blood samples, especially in dogs suspected of being positive by 2ME-MPAT, but negative by a blood culture test.

Article Info

Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 043, Issue 5, Dec 09 - Dec 09, Page 159 - 164 |  PDF |  Page